منصة إيفاد العلمية

Shadia Taghian Ahmed Mohamed

إرسال رسالة

التخصص: Plant pathology

الجامعة: Agreculture Research Institute ,Giza ,Egypt

النقاط:

معامل الإنتاج البحثي

الخبرات العلمية

Isolation of microorganisms from sugar beet phyllosphere. Isolation endophytic bacteria from sugarcane and other plant Molecular biology techniques: PCR. Experience in tissue culture techniques in sugarcane and stevia plants. Isolation endophytic bacter

الأبحاث المنشورة

SYSTEMIC RESISTANCE IN SUGAR BEET ELICIATED BY NON-PATHOGENIC, PHYLLOSPHERE-COLONIZING Bacillus pumilus AND B. subtilus AGAINST THE PATHOGEN Cercospora beticola Sacc.

المجلة: J. Plant Prot. and Path., Mansoura Univ., Vol. 2 (1): 67 - 83, 2011

سنة النشر: 2011

تاريخ النشر: 2011-01-01

A total of 10 Bacillus subtilis (7) and Bacillus pumilus (3) isolates from sugar beet phyllosphere were evaluated for its biocontrol activity against sugar beet cercospora leaf spot disease (CLS) under greenhouse and field conditions compared to the fungicide Topsin M70. PR-related proteins, chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase as well as indol acetic acid were determined in treated and untreated sugar beet plants. Under greenhouse conditions the reduction in disease severity caused by B. subtilis and B. pumilus treatments ranged from 73.33 - 80% and 80 - 83% respectively. Under field conditions the reduction ranged from 56.18 to 81.27% and 62.55 to 81.27% from B. subtilis and B. pumilus respectively. The levels of the determined PR proteins significantly increased in all the treated plants either with B. subtilis or B. pumilus. In addition, the levels of IAA were higher in the treated plants compared to the untreated plants. The sucrose percentage increased in the treated plants compared to the infected untreated control. The present study suggested the role of Bacillus subtilis and Bacillus pumilus in inducing plant acquired resistance.

Role of Emericella nidulans and Epicoccum nigrum in Controlling Sugar Beet Leaf Spot Disease

المجلة: Egyptian-Arab J. Applied Sci. and Tech. (EAJAST) (2021) 1(2):55-65 Issued by The Egyptian Arab Foundation for Investment, Innovation and Industrial Development (EAFID) 1

سنة النشر: 2021

تاريخ النشر: 2021-10-13

Two fungal isolates from commercial sugar beet phyllospher in Kafr-Elshaikh governorate, Egypt were tested for its antagonistic activity against Cercospora beticola the causal pathogen of sugar beet cercosporia leaf spot (CLS) disease in vitro. Three methods were used to evaluate its antagonistic activity (Conventionalstreak, culture filtrates and volatile gases). The ability of the tested isolates to produce PR-proteins and growth-promoter indole acetic acid were studied. The tested isolates Emericella nidulans (Aspergillus nidulans) and Epicoccum nigrum found to have the potential to produce chitinase and β-1, 3 glucanase while Emericella nidulansthe only found to have the potential to produce indole acetic acid (IAA). The tested fungal isolates showed a remarkable antagonistic activity in both green-house and open field against C. beticola compared with the healthy control and the infected control. Also it was found that the control efficiency of the two isolates was almost equivalent to the tested fungicide. The present data show that phyllospheric fungi can play as a good candidate to be used as a bio-control agent against sugar beet leaf spot disease.

BACILLUS SUBTILIS AS BIOAGENT USED TO CONTROL CERCOSPORA SUGAR BEET LEAF SPOT DISEASE

المجلة: Zagazig J. Agric. Res., Vol. 35 No. (6) 2008

سنة النشر: 2008

تاريخ النشر: 2008-12-17

In Egypt, Cercospora beticola Sacc., causes leaf-spot, the most serious diseases on sugarbeet, that dramatically reduces the yield quantity and quality. The availability of fungicides from different classes has become a crucial component in controlling the disease due to environmental hazards. Twenty one Bacillus subtilis isolates previously isolated from sugarbeet phyllospher at different Egyptian governorates significantly reduced Cercospora leaf spot (CLS) severity compared to the untreated control plants under greenhouse and field conditions for three successive growing seasone (2005/2007). Data showed that non significant difference was detected between the tested B. subtilis isolates and the fungicide treatment. Data of the third season revealed that the lowest severity was recorded with SHR-27-5 isolate (0.5%) while the highest severetity (1.0%) was detected with DK-36-3B and KSH-43-15 isolates compared to the untreated control treatment (2.67%). Data also showed that disease reduction using some selected B. subtilis isolates ranged from 62.55-74.91%. The present study showed the efficiency and importance of using bioagent active isolates of B. subtilis for controlling CLS disease on sugar beet in Egypt.

ENDOPHYTIC BACTERIA FOR BIOLOGICAL CONTROL OF SUGARCANE SMUT DISEASE AND ITS EFFECT ON SOME PLANT GROWTH PARAMETERS

المجلة: Egyptian-Arab J. Applied Sci. and Tech. (EAJAST) (2021) 1(2):39-53

سنة النشر: 2021

تاريخ النشر: 2021-08-16

In the is study, the endophytic bacteria were isolated from sugarcane stalks and tested for its in vitro antagonistic activity against Sporisorium scitamineum, the causal pathogen of sugarcane smut disease. Also some physiological characteristics such as its ability to produce some secondary metabolites i.e. PR- proteins and growth-promoters were studied in both in vitro and in vivo. Only, 62 isolates among the total 240 isolates were found to have a bioactivity against the pathogen in vitro. Six isolates from the bioactive isolates were selected for its high antagonistic activity to study its potential to produce the enzymes (chitinase, and β 1,3 glucanase,) and growth-promoters (indole acetic acid (IAA) as well as siderophores and salicylic acid (SA)) in vitro. The selected isolates showed different degrees of antagonistic activity. The selected isolates were identified using the sequencing of the 16S ribosomal RNA (rRna) gene and subjected to mega blast-n in NCBI and proved to be Enterobacter sp.( LUX 27), Kosakonia radicictans (LUX41), Kosakonia radicictans(BAN 33), Klabsiella oxytoca (BAN 39), Bacillus subtilis subsp. Inaquosorum(Q17), Pantoea sp.(SOH 29). In vitro studies proved that, all selected endophytic bacteria produced Nitrogenase and indole acetic acid (IAA) while, Kosakonia radicictans, Klabsiella oxytoca and Bacillus subtilis subsp. Inaquosorum produced salicylic acid. Not one of the selected isolated prove to produce Siderophores. Pantoea sp., Kosakonia radicictans and Klabsiella oxytoca produced chitinase. While, the isolates Kosakonia radicictans and Bacillus subtilis subsp. Inaquosorum were the most effective in producing β 1,3glucanase. The bio-control activity of selected isolates was also studied in vivo under greenhouse conditions on sugarcane infected plants. All the tested isolates were completely reduced the disease compared to the infected control. All the selected endophytic bacteria increased Peroxidase, Chitinase and β-1, 3 glucanaseactivities in the treated plants while the isolates Kosakonia radicictans and Enterobacter sp. didn’t produce Polyphenol-oxidase in treated infected plants. The treated sugarcane plants showed a range of positive increase in Leaf Area, Stalk length, Stalk Diameter, number of tillers and total chlorophyll compared to the untreated healthy and infected control. The present study show the efficiency of using endophytic bacteria as a bio-control system against sugarcane smut.

ANTAGONISTIC ACTIVITIES OF Bacillus amyloliquefaciens FROM PHYLLOSPHERE OF SUGAR BEET AGAINST Cercospora beticola sacc.

المجلة: Journal of Plant Protection and Pathology

سنة النشر: 2011

تاريخ النشر: 2011-01-01

ENDOPHYTIC BACTERIA ASSOCIATED WITH SUGARCANE AND ITS POTENTIAL TO SUPPRESS SPORISORIUM SCITAMINEUM, THE CAUSAL FUNGAL PATHOGEN OF SMUT DISEASE

المجلة: 14 th Conf. Agric. Develop. Res., Fac. of Agric., Ain Shams Univ., March, 2019, Cairo, Egypt Special Issue, 27(1), 825 – 836, 2019 Website: http://strategy-plan.asu.edu.eg/AUJASCI

سنة النشر: 2019

تاريخ النشر: 2019-03-10

Bacterial endophytic ubiquitously colo- nize the internal tissues of the plant. The pur- pose of this study was to isolate and identify the endophytic bacteria from sugarcane stalks which have antagonistic activity against Sporisorium scitamineum, the causal patho- gen of sugarcane smut disease, and to study their activity to produce some secondary me- tabolites i.e. enzymes and growth-promoters. Sugarcane samples were collected from five governorates in Upper-Egypt namely, Giza, Beni-Suef, Sohag, Qena, and Luxor. A total of 240 isolates of endophytic bacteria were iso- lated from 160 samples of healthy sugarcane stalks (variety GT-54-9). Isolated endophytes were screened for its antagonistic activity against S. scitamineum, in vitro, using dual culture method. Only, 62 isolates showed different degrees of antagonistic activity. Ten isolates of endophytic bacteria were selected to study their potential to produce enzymes (chitinase, β 1,3 glucanase, phenylalanine ammonia lyase) and growth-promoters (si- derophores, indole acetic acid (IAA), salicylic acid (SA)). These isolates were pre-identified using the morphological and physiological properties according to Bergy's manual as Pseudomonas sp., Serratia sp., Enterobacte- ria sp., Herbaspirillium sp., Gluconacetobac- ter sp. Anabaenopsis sp. and Azospirillumsp. All endophetic bacteria produced β 1,3gluconase and indole acetic acid (IAA), while Pseudomonas sp. produced salicylic acid and phenylalanine ammonia lyase, Ana- baenopsis sp. produced phenylalanine am- monia lyase, Gluconacetobacter sp. produced chitinase and Herbaspirillium sp. produced siderophore. Meanwhile, isolates of Anabae- nopsis sp. and Pseudomonas sp. were the most effective to produce β 1,3glucanase and indole acetic acid compared with other ento- phytic bacteria.

Phyllispher microflora of sugar beet and their interaction with cercospira beticola the causal agent of cercospira leaf spot disease

المجلة: Egypt.J.of.Apple.Sci.,25(8B)2010

سنة النشر: 2010

تاريخ النشر: 2010-01-01

Effect of leaf surface fungal isolates in controlling cercospora leaf spot of sugar be.

المجلة: . Egypt J.appl.Sci.26:13-37

سنة النشر: 2011

تاريخ النشر: 2011-11-01