منصة إيفاد العلمية

سوزان عبد الجبار عبد العزيز

إرسال رسالة

التخصص: علوم الحياة-الطفيليات

الجامعة: البصرة

النقاط:

10.5

معامل الإنتاج البحثي

الخبرات العلمية

تشخيص وتصنيف الطفيليات على المستوى الشكلي والجزيئي
تشخيص وتصنيف الطفيليات على المستوى الشكلي والجزيئي
الكشف المناعي عن الاصابات الطفيلية في الانسان والحيوان
الكشف الجيني الوراثي لسلالات انواع متعددة من الطفيليات المشتركة بين الانسان والحيوان

الأبحاث المنشورة

Evaluation of the Sensitivity of Three Immunological Diagnostic Techniques for the Diagnosis of Toxoplasmosis in Aborted Women in Kirkuk/ IRAQ

المجلة: Passer Journal

سنة النشر: 2024

تاريخ النشر: 2024-05-13

Background: Toxoplasmosis is widely recognized as a highly widespread parasitic infection in humans, capable of vertical transmission from mother to fetus. Clinical problems associated with toxoplasmosis during pregnancy include spontaneous miscarriage, premature labor, stillbirth, and fetal abnormalities. Objective: to evaluate the sensitivity of three immunological methods for the diagnosis of Toxoplasma. gondii in serum and placental tissue of aborted women. Materials and Methods:80 blood and placental Specimens were obtained from women who underwent abortion and were admitted to the Maternity ward and Child hospital in Kirkuk City. Blood samples were analyzed for anti-Toxoplasma antibodies IgM and IgG using ELISA and Combo Rapid test and placental samples were tested for T. gondii antigen in the placental tissue using the Immunohistochemistry technique. Results: The presence of anti- T. gondii antibodies in women who have undergone abortions was 30% (IgG) when the Combo Rapid test was used and 16.3% (IgG) when ELISA was used. In all cases, neither the combo rapid test nor the ELISA demonstrated seropositivity of IgM. IHC examination revealed that 60% of tissues had toxoplasmosis infection, whereas 40% of tissues were negative. Conclusion: the finding of this study indicates that IHC is a more sensitive immunological technique than a serological assay for the direct diagnosis of T. Gondii in the placental tissue of aborted women.

Molecular Prevalence and Genotyping of Toxoplasma gondii from Pet Cat Using PCR-RFLP

المجلة: BASRAH JOURNAL OF VETERINARY RESEARCH

سنة النشر: 2024

تاريخ النشر: 2024-06-01

Toxoplasma gondii is the most dangerous parasite found in humans and cats. The strong relationship between cats and their owners compelled us to explore the incidence of these parasites in companion cats and genotyping of T.gondii strain of the infected pets. 54 blood samples and 54 fecal samples collected from pets in order to ascertain the prevalence of T. gondii. The GRA6 gene was targeted in the feces and blood of examined pets and the genotypes of T. gondii were determined using the GRA6 gene loci by PCR-RFLP assay. The GRA6 gene amplification showed that 6% of pets were infected with T.gondii. PCR-RFLP assay revealed the predominance of genotype II at a rate 67% and type III at a rate 33% while type I not detected in infected pets. The incidence of toxoplasmosis in domestic felines can cause contamination of environment through excreting of oocyst in the feces which may lead to infection to cat owners specially who taking care of feeding and cleaning of pets.

Molecular Identification of Fasciola gigantica in Cattle by Using a Conventional PCR in Basrah Governorate

المجلة: BASRAH JOURNAL OF VETERINARY RESEARCH,

سنة النشر: 2024

تاريخ النشر: 2024-03-02

Fascioliasis represents an ignored zoonotic disease that results from liver flukes that belong to the genus Fasciola spp. It results in substantial financial damages in the cattle sector. In the current investigation, liver flukes were taken from cattle in two places. The Basrah abattoir and the Aljazeera region. The flukes were found in buffalo and a cow. The nuclear marker genes were used to identify the species and learn about the past of Fasciola spp. spread. From a total number of examined Buffalo (11) and Cow (11), the infection with Fasciola spp. were in Buffalo and in Cow the percentage of infection was 18.18 % (2/11), 81. 82% (9/11) respectively. The Polymerase chain reaction results showed that all examined samples by PCR were found as Fasciolagigantica, with an identity of 100% according to GenBank. In the current study, we isolated Fasciola spp. Two worms from sheep and PCR were done, and it showed that it is F. gigantica. This indicates that the distribution of the parasite is emerging from the host specificity, which means it might be molecularly done in detail studies in all types of cattle, not only in Basrah city but all over Iraq.

Active survey of hydatid cysts in slaughtered sheep at Basrah abattoirs, Basrah province, Iraq

المجلة: Journal of Entomology and Zoology Studies

سنة النشر: 2017

تاريخ النشر: 2017-10-07

The current study focused on slaughtered sheep in Basrah city which infected with hydatid cysts from the period between June 2016 till February 2017. The total sheep examined 1145 and169 were found to be positive for hydatidosis. In current study, the prevalence was estimated to be 14.75, and the female sheep 123 (22.9) was found more infected with hydatid cysts than sheep male 46 (7.5). The highest number of infected sheep with hydatid cysts was observed in July and August while the lowest number was observed between September and October. Distribution of cystic hydatidosis according to the cyst localization indicated the livers and lungs were the most frequently infected visceral organs in sheep. The findings of this study demonstrated that hydatid cysts are common and widely distributed in sheep and they might play an important role in the life cycle and transmission of this zoonosis in Basrah province.

Monthly variation of the parasiting on carangid fishes in northwest of the Arab Gulf, Iraq

المجلة: Global Journal of Fisheries and Aquaculture Researches

سنة النشر: 2018

تاريخ النشر: 2018-11-12

A total number of 600 fishes were collected between September 2011 to March 2012 from Iraqi marine waters north west the Arab Gulf. These fishes were represented ten species: Alectis indica, Alepes djedaba, A. kleinii, Atropus atropos, Atule mate, Carangiodes armatus, C. bajad, C. malabaricus, Gnathanodon speciosus and Megalaspis cordyla. The result of inspection showed that 107 fish species were infested with nine species of parasites. These included five species of trypanorhynch cestodes: Callitetrarhynchus gracilis, Callitetrarhynchus sp., Floriceps minacanthus, Progrillotia sp., Pseudogrillotia spratti, and one species of larva nematode Skrjabillanus sp. Also three species of copepods Lernanthropus indicus, L. corniger and Caligus cordyla. The present study revealed that the highest prevalence of infection in M. cordyla (93.7%) and the lowest prevalence in C. malabaricus (6.25%). The present study reports that the highest mean intensity of the parasite was in cestode (4.65) and the lowest occurrence in nematode (1.00).