Evaluation of the efectiveness of some mycorrhizal fungi isolates against charcoal rot disease

المجلة: Egyptian Journal of Biological Pest Control

سنة النشر: 2023

تاريخ النشر: 2023-10-12

The sunfower plants are attacked by serious seed and soil-borne pathogens including charcoal rot dis- ease that caused by Macrophomina phaseolina. This disease has serious damages to sunfower crop. This study aimed to assess the efcacy of Arbuscular mycorrhizal fungus against charcoal rot disease as fungicide alternative. Results Morphological and molecular identifcation was done, using universal primers for molecular identifcation. Finally, a greenhouse experiment was conducted, and the length and weight of the plant shoot and root as well as disease incidence and severity percentages were estimated for the treatments infected with the M. phaseolina pathogen. The results showed that molecular identifcation indicates their subordination to Claroideoglomus etuni- catum and Funneliformis mosseae. The greenhouse experiment showed that combined inoculation treatments (C. etunicatum+F. mosseae and C. etunicatum+F. mosseae+M.phaseolina) had the highest averages of length and weight of the plant shoot and root in the non-infected and pathogen-infected (M. phaseolina) treatments. F. mosseae and F. mosseae+M.phaseolina treatments had better efects on plant growth. M. phaseolina pathogen had suppression efects than the C. etunicatum inoculum (C. etunicatum and C. etunicatum+M. phaseolina treatments). The disease incidence and severity percentages decreased signifcantly in the inoculated plants than in non-inoculated plants. F. mosseae inoculum showed more efciency in reducing DI and DS than C. etunicatum inoculum, while adding both, resulting in an extra signifcant reduction. The combined inoculation, which functions as a biofungicide and a bioferti- lizer, presented a viable biocontrol technique for crop protection. Conclusions Such the AM fungi treatments are considered good and biological safe. The symbiotic relationship of AM fungi with plants can also be utilized to gain successful management of agricultural against soil-borne diseases for getting rid of the negative efects of pesticides