منصة إيفاد العلمية

سميرة موسى ساسي

إرسال رسالة

التخصص: علم الاجنة

الجامعة: طرابلس

النقاط:

22.5

معامل الإنتاج البحثي

الخبرات العلمية

الأبحاث المنشورة

Effect of Dimethoate Insecticide on Female Mice and Their Fetuses

المجلة: J Dent Med Res.

سنة النشر: 2024

تاريخ النشر: 2024-02-03

Organophosphate insecticides (OPIs) including dimethoate are used in agriculture to control the insect pests, increasing the productivity, their frequent use has resulted in detrimental effects on biological systems and public health. This study aimed to evaluate the effect of dimethoate (DM) on fetuses, DNA concentration in ovarian tissues and levels of estradiol and progesterone in female mice. Methods. Female mice were divided into three groups: Group I, serve as control and was given distilled water intraperitoneally, while Group II and III were given (0.1- & 0.2-ml DM/100 ml distilled water) respectively for 7 days. Two treated females were housed with one untreated male for mating. After 7 days of the last dose, 6 female mice of each group were weighed and killed. The ovaries, uteri and liver were extracted and weighed, and ovarian hormones were measured. Also, DNA concentration in each ovary was determined. Results. The results showed a significant decrease in the weights of the liver and ovaries, the level of estradiol hormone, and DNA concentration in the ovary of treated mice as compared to those from the control group. Also, significant changes were observed in the weights of fetuses as well as the number of live fetuses. Conclusion. We found that administration of DM intraperitoneally to female mice for 7 days has influences on ovary weight, estradiol levels, and DNA concentration as well as its impact on the fetuses, therefore, it is better to use safer methods for control the pests and avoid increased use of pesticides.

Ciprofloxacin Affects Sperm Quality and Induces Testicular and Hepatic Injury in Male Mice

المجلة: Alq J Med App Sci

سنة النشر: 2024

تاريخ النشر: 2024-07-07

Ciprofloxacin (CPFx) is one of a second-generation fluoroquinolones antibiotic and is prescribed a lot for curing bacterial infection. The aim of this study was to investigate of impact CPFx on sperm characteristics, liver DNA and histological structure of liver and testes. Twenty-four male mice aged 7-10 weeks were divided into three groups of eight. The first group (control) was given distilled water. The second and third groups received CPFx with doses 500 and 750 mg/kg respectively. All treatments were administrated orally for 14 days. Three untreated females were put with one treated male for mating. At the end of dosing period, mice were killed, testes and liver were removed and weighed. Sperm parameters, testosterone level and liver DNA were measured. Histopathology of liver and testes was carried out. The results showed that CPFx significantly affected liver DNA, sperm motility and morphology as well as it caused histopathological alterations of hepatic and testicular tissues. Moreover, it led to a significant elevation in the percentage of dead fetuses and also deformity fetuses. The results indicate that CPFX has detrimental effects on sperm quality, liver DNA and histological structure of testes and liver; therefore, it should be used with great caution.

Positive effect of green tea extract on reproductive toxicity induced by dimethoate in male mice

المجلة: Open Veterinary Journal,

سنة النشر: 2022

تاريخ النشر: 2022-03-03

Background: Dimethoate (DM) is one of the most organophosphorus pesticides used all over the world to control insect pests, the extensive use of this insecticide causes a health hazard to animals and humans. Aim: This study was conducted to evaluate the positive effect of green tea extract on sperm quality and testicular cytoarchitecture in male mice treated with DM and on its reproductive performance. Methods: Mice were divided into three groups, each group contained nine mice, the first group (control) was given distilled water only, the second group received DM at a dose (0.1 ml DM/100 ml distilled water) while the third group was given DM at a dose (0.1 ml DM/ 100 ml distilled water) and the green tea extract at a dose (100 mg/kg). After 20 days of the treatment, six mice from each group were killed, sperm quality (sperm count, morphology motility) and histopathological lesions of testis were evaluated. Results: The results showed that DM significantly affected sperm quality a decrease in sperm motility and an increase in abnormal sperm morphology and caused marked alterations in the microstructures of testicular tissues. When treated males were mated with untreated females, a decline in the number of live embryos was found, while the green tea extract revealed an effective role by reducing those negative influences. Conclusion: This study revealed that DM has detrimental effects on sperm quality, testicular tissues, and the embryos, while treatment with green tea revealed a positive role in improving those negative influences of DM without causing any harmful side effects

Sperm abnormality and infertility in male mice treated with the recommended dose of dimethoate and its double

المجلة: Open Veterinary Journal,

سنة النشر: 2023

تاريخ النشر: 2023-07-31

Background: Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms. Aim: This study was conducted to evaluate the effect of the recommended dose and its double of DM on sex hormones, sperm morphology, and fertility of adult male mice. Methods: Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM/100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1:2). Result: The results revealed a decrease in luteinizing hormone levels in mice treated with (0.2 ml DM/100 ml distilled water) compared with the control group while the levels of follicle-stimulating hormone and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility was reduced and the average number of dead embryos increased while the average number of live embryos decreased. Conclusion: This current study confirmed that DM has detrimental effects on sperm morphology, fertility, and the embryos; therefore, more efforts should be exerted to protect ourselves and our environment from the harmful effects of this pesticide.

Effect of Peganum harmala seeds extract on the hepatic tissue structure and fetus of mice

المجلة: Ovozoa : J Anim Reprod

سنة النشر: 2023

تاريخ النشر: 2023-12-01

Peganum harmala is one of the most used plants for the treatment of many diseases. Its effective compounds have pharmaceutical and medicinal properties. This study aims to determine the effect of aqueous extract of harmala plant seeds on body and liver weight, aspartate aminotransferase (AST), alanine transaminase (ALT), and the histological structure of liver of mice, as well as the size of the fetuses sired by treated mice. Sixteen adult male mice were divided into two groups of eight. The first group (control) was given distilled water orally, while the second group received the aqueous extract of harmala seeds at a dose of 300 mg/kg bw for three weeks. three untreated females were housed with one treated male for mating. At the end of the treatment, six male mice of each group were weighed and killed. Liver was extracted, weighed and its enzymes were measured. Also, sections of the liver were prepared for histological examination. The results showed a decrease in the body weight of the treated mice and a significant increase in the average weight of the fetuses compared with the control group, as well as marked changes in the hepatic tissue structure. There was no impact of the extract on fetal body length, liver weight and hepatic enzymes (AST and ALT) of treated mice. Further studies should be conducted to determine a safe dose that does not affect any organ in the body, so that it can be used for the treatment of many diseases

Prophylactic and Curative Effect of Boswellia Serrata on Blood Glucose Level and Architecture of Pancreas in Diabetic Mice Induced by Alloxan

المجلة: Khalij J Dent Med Res.

سنة النشر: 2023

تاريخ النشر: 2023-12-05

Background and objectives. Herbal medicines have gained significant attention from the scientific community for treating and managing diabetes in both developing and developed countries. Boswellia serrata, known for its anti-inflammatory and antioxidant properties, has been historically used to treat various diseases. This study aimed to determine the effect of Boswellia serrata on body weight, blood glucose level, and pancreas architecture in alloxan-induced diabetes in albino mice. Methods. Forty albino male mice, aged 10 to 12 weeks and weighing between 24 and 34 grams, were divided into four groups of ten each. The first group was the negative control group, and the second group was the diabetic positive control group that received 120 mg/kg alloxan intraperitoneally. The third group received a single injection of alloxan at a dose of 120 mg/kg bw and was treated with Boswellia serrata macerated at a dose of 150 mg/kg bw for four weeks. The fourth group received orally Boswellia serrata extract at a dose of 150 mg/kg for two weeks, followed by a single injection of alloxan at a dose of 120 mg/kg, and were left for two weeks. Results. This study found that Boswellia serrata has antidiabetic properties in diabetic mice, as it significantly reduced blood sugar levels compared to the diabetic group. Additionally, histological analyses of the groups treated with B. serrata showed a significant improvement in the harmful effects of alloxan on pancreatic islet cells. Conclusion. Boswellia serrata has shown a notable improvement in the detrimental effect of alloxan on pancreatic islet cells, and its curative effect has been proven to be better than its prophylactic effect. This is supported by the improved blood glucose levels and the histological changes that were more pronounced.

Carbon Tetrachloride-Induced Testicular Toxicity and Histopathological Alteration in Male Swiss Albino Mice

المجلة: Alq J Med App Sci.

سنة النشر: 2024

تاريخ النشر: 2024-01-11

Recently there has been an increased association between toxic substances present in the environment and male infertility. Carbon tetrachloride (CCL4) is widely used as a chemical intermediate and as a feedstock in the production of chlorofluorocarbons. CCL4 is highly toxic to the liver, kidney, testicle, brain and other tissues. Therefore, the present study was designed to identify its effect on the reproductive system in adult male mice. Thirty adult male albino mice were divided into three equal groups (n=10): the first group control, the second group received 0.1m/100g body weight olive oil and the third group received 0.1ml/100g/body weight CCL4 intraperitoneally every alternate day for three weeks. The results showed that CCl4 causes a significant decrease in body weight, sperm motility, sperm count and testosterone level, while it leads to a significant increase in the number of abnormal sperm morphology. Additionally, CCL4 caused apparent alterations in the histological structure of the testes. In conclusion, CCl4-induced reproductive toxicity in male mice.

Sperm Kinematics and Morphology of Bali Bull (Bos javanicus) after Freezing and Thawing Treated with Green Tea Extract in Extender

المجلة: Tropical Animal Science Journal

سنة النشر: 2023

تاريخ النشر: 2023-08-18

Green Tea Extract, or GTE, is a powerful antioxidant that can be added to the extender to maintain the quality of Bali bull semen before and after freezing. This study aimed to evaluate the impact of the GTE addition in the extender on the sperm kinematic and sperm morphology from frozen semen of Bali bull. Five Bali bulls from the NAIC, were used as semen sources. In this study, there were four different treatments: a control (Tris-egg yolk without GTE), a GTE0.05 (100 mL tris egg yolk with 0.05 mg of GTE), a GTE0.10 (100 mL tris egg yolk with 0.10 mg of GTE), and a GTE0.15 (100 mL tris egg yolk with 0.15 mg of GTE). A sperm analyzer (CASA) was used to observe the sperm kinematics and morphology. Compared to the control group, adding different concentrations of GTE to the semen extender did not affect the sperm’s morphology. Before freezing, adding GTE0.05 increases total and progressive motility as well as VAP, VCL, and VSL after thawing. GTE0.15 increases STR and ALH. The conclusion is that GTE additions at doses of 0.05 mg/100 mL and 0.15 mg/100 mL can increase sperm kinematics parameters, but sperm morphology is not affected by the GTE.

Zinc and α-tocopherol protect the antral follicles and endogenous antioxidants of female albino rats (Rattus norvegicus) against lead toxicity

المجلة: Journal of Trace Elements in Medicine and Biology

سنة النشر: 2023

تاريخ النشر: 2023-08-18

Background: Lead impairs female reproductive health because it can induce oxidative stress. Zinc as an antioxidant produces an enzyme system that helps neutralize free radicals. α-Tocopherol has an antagonistic effect that reduces oxidative stress. This study aimed to demonstrate the effects of zinc (Zn) and α-tocopherol on the ovarian endogenous antioxidants and antral follicles of albino rats (Rattus norvegicus) exposed to lead acetate (Pb (C2H3O2)2). Methods: Twenty-five female Wistar rats were divided into five groups, namely groups K (control), P0, P1, P2, and P3. Following exposure and treatment for 21 days with different combinations, the albino rats were necropsied, and their ovaries were removed for subsequent histopathological preparations and endogenous antioxidant analysis. Observations were made on the ovary, including an antral follicle count and diameter calculations. Analysis of the superoxide dismutase (SOD) levels (560 nm wavelength) and malondialdehyde MDA-TBA (532 nm wavelength) were performed by a spectrophotometer. The data were analyzed using a oneway ANOVA and least significant difference (LSD) test with the SPSS V24 software. Results: The highest SOD enzyme expression in the albino rat ovaries was in P0 (17.23 ± 5.34), and the lowest was in P3 (4.21 ± 0.76). The lowest MDA level was observed in the control group (K) and P3 compared to the other groups. The highest average antral follicle count and diameter were found in the albino rats exposed to 1.5 mg/kg BW lead acetate, and treated with 0.54 mg/kg BW zinc sulfate and 100 mg/kg BW α-tocopherol (group P3) compared to the other groups. The mechanisms of action of zinc and α-tocopherol work synergistically to decrease free radicals and ovarian damage. Conclusion: The results showed that a combination of 0.54 mg/kg BW zinc (Zn) and 100 mg/kg BW α-tocopherol can maintain the number and diameter of the antral follicles and reduce ovarian SOD expression and MDA levels in albino rats exposed to lead acetate.