ملف المستخدم
صورة الملف الشخصي

ناجية موسى ساسي الغول

إرسال رسالة

التخصص: مكافحة حيوية

الجامعة: طرابلس

النقاط:

24
معامل الإنتاج البحثي

الخبرات العلمية

الأبحاث المنشورة

Positive effect of green tea extract on reproductive toxicity induced by dimethoate in male mice

المجلة: Open Veterinary Journal

سنة النشر: 2022

تاريخ النشر: 0022-03-03

Background: Dimethoate (DM) is one of the most organophosphorus pesticides used all over the world to control insect pests, the extensive use of this insecticide causes a health hazard to animals and humans. Aim: This study was conducted to evaluate the positive effect of green tea extract on sperm quality and testicular cytoarchitecture in male mice treated with DM and on its reproductive performance. Methods: Mice were divided into three groups, each group contained nine mice, the first group (control) was given distilled water only, the second group received DM at a dose (0.1 ml DM/100 ml distilled water) while the third group was given DM at a dose (0.1 ml DM/ 100 ml distilled water) and the green tea extract at a dose (100 mg/kg). After 20 days of the treatment, six mice from each group were killed, sperm quality (sperm count, morphology motility) and histopathological lesions of testis were evaluated. Results: The results showed that DM significantly affected sperm quality a decrease in sperm motility and an increase in abnormal sperm morphology and caused marked alterations in the microstructures of testicular tissues. When treated males were mated with untreated females, a decline in the number of live embryos was found, while the green tea extract revealed an effective role by reducing those negative influences. Conclusion: This study revealed that DM has detrimental effects on sperm quality, testicular tissues, and the embryos, while treatment with green tea revealed a positive role in improving those negative influences of DM without causing

Sperm abnormality and infertility in male mice treated with the recommended dose of dimethoate and its double

المجلة: Open Veterinary Journal

سنة النشر: 2023

تاريخ النشر: 0023-02-03

Background: Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms. Aim: This study was conducted to evaluate the effect of the recommended dose and its double of DM on sex hormones, sperm morphology, and fertility of adult male mice. Methods: Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM/100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1:2). Result: The results revealed a decrease in luteinizing hormone levels in mice treated with (0.2 ml DM/100 ml distilled water) compared with the control group while the levels of follicle-stimulating hormone and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility was reduced and the average number of dead embryos increased while the average number of live embryos decreased.

‫Effect of Early Exposure of Dimethoate on Reproductive Potential in Swiss Male Mice J

المجلة: المجلة السورية للبحوث الزراعية

سنة النشر: 2018

تاريخ النشر: 2018-12-06

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Effect of Early Exposure of Dimethoate on Reproductive Potential in Swiss Male Mice

المجلة: المجلة السورية للبحوث الزراعية

سنة النشر: 2018

تاريخ النشر: 2018-12-06

Abstract: This study was done to evaluate reproductive potential of male mice following early exposure to dimethoate. Thirty-six Swiss albino male mice were divided into three groups of twelve mice each: first group injected intraperitoneally with distilled water, and used as a control group, while second and third groups injectedwith dimethoate at doses of 0.1 and 0.2 ml dimethoate/100 ml distilled water,respectively for 7 days. At the end of experiment, the mice were weighed and thenwere killed. Testis and epididymis weights were recorded, sperm parameters wereevaluated. The results revealed a decrease in body and testis weights in secondgroup treated with dimethoate, while epididymis weights did not affect, also the statistical analysis showed a significant decrease in sperm motility and increase of abnormal sperm in dimethoate treated mice compared to control group, while sperm count did not show any effect, also marked alterations in microstructure of testicular tissues observed. In addition, there was a significant increase in mean percentage of dead embryos and deformed embryos of untreated females mating with dimethoate treated males compared to control, there was no effect of pesticide on the mean weight of embryos. This study emphasized that the dimethoate hasharmful effect on testis, sperm parameters and the embryos. Therefore, more efforts should be exerted to protect our environment and health from seriously harmful effects of this insecticide.

Effect+of+Peganum+harmala+seeds+extract+on+the+hepatic+tissue+structure

المجلة: Ovozoa

سنة النشر: 2023

تاريخ النشر: 2023-12-12

Peganum harmala is one of the most used plants for the treatment of many diseases. Itseffective compounds have pharmaceutical and medicinal properties. This study aims to determinethe effect of aqueous extract of harmala plant seeds on body and liver weight, aspartateaminotransferase (AST), alanine transaminase (ALT), and the histological structure of liver ofmice, as well as the size of the fetuses sired by treated mice. Sixteen adult male mice were dividedinto two groups of eight. The first group (control) was given distilled water orally, while the secondgroup received the aqueous extract of harmala seeds at a dose of 300 mg/kg bw for three weeks.three untreated females were housed with one treated male for mating. At the end of the treatment,six male mice of each group were weighed and killed. Liver was extracted, weighed and itsenzymes were measured. Also, sections of the liver were prepared for histological examination. Theresults showed a decrease in the body weight of the treated mice and a significant increase in theaverage weight of the fetuses compared with the control group, as well as marked changes in thehepatic tissue structure. There was no impact of the extract on fetal body length, liver weight andhepatic enzymes (AST and ALT) of treated mice. Further studies should be conducted to determinea safe dose that does not affect any organ in the body, so that it can be used for the treatment ofmany diseases (PDF) 3 +Effect+of+Peganum+harmala+seeds+extract+on+the+hepatic+tissue+structure. Available from: https://www.researchgate.net/publication/376384777_3_EffectofPeganumharmalaseedsextractonthehepatictissuestructure#fullTextFileContent [accessed Aug 24 2024].

Effect of Dimethoate Insecticide on Female Mice and Their Fetuses

المجلة: KHALIJ-LIBYA JOURNAL

سنة النشر: 2024

تاريخ النشر: 2024-10-03

Background and objectives. Organophosphate insecticides (OPIs) including dimethoate are used in agriculture to control the insect pests, increasing the productivity, their frequent use has resulted in detrimental effects on biological systems and public health. This study aimed to evaluate the effect of dimethoate (DM) on fetuses, DNA concentration in ovarian tissues and levels of estradiol and progesterone in female mice. Methods. Female mice were divided into three groups: Group I, serve as control and was given distilled water intraperitoneally, while Group II and III were given (0.1- & 0.2-ml DM/100 ml distilled water) respectively for 7 days. Two treated females were housed with one untreated male for mating. After 7 days of the last dose, 6 female mice of each group were weighed and killed. The ovaries, uteri and liver were extracted and weighed, and ovarian hormones were measured. Also, DNA concentration in each ovary was determined. Results. The results showed a significant decrease in the weights of the liver and ovaries, the level of estradiol hormone, and DNA concentration in the ovary of treated mice as compared to those from the control group. Also, significant changes were observed in the weights of fetuses as well as the number of live fetuses. Conclusion. We found that administration of DM intraperitoneally to female mice for 7 days has influences on ovary weight, estradiol levels, and DNA concentration as well as its impact on the fetuses, therefore, it is better to use safer methods for control the pests and avoid increased use of pesticides.

Effect of The Recommended Dose of Dimethoate and Its Double on The Embryos and Testicular Tissues in Male Mice

المجلة: مجلة علوم البحار والتقنيات البيئية

سنة النشر: 2021

تاريخ النشر: 2021-12-06

Exposure to pesticides negatively have associated with many public health hazards infecting humans as infertility, therefore, the objective of this study was to investigate the effect of dimethoate on testicular cytoarchitecture of treated mice and on the embryos. Albino mice were divided into three groups of 9 each: the first group served as control and was given distilled water orally, whereas the second and third groups were given dimethoate at doses (0.1 and 0.2 mL dimethoate/100 mL distilled water) respectively for 20 days. At the end of the treatment, six mice from each group were weighed and sacrificed, the testis and epididymis weights were recorded, and histopathological lesions of tests were carried out. The obtained results revealed that dimethoate caused a decrease in the body and epididymis weights. Furthermore, dimethoate led to a significant decrease in the number of dead embryos and their weights. Histopathological examination demonstrated that this pesticide caused marked alterations in the microstructure of testicular tissues appeared as vacuoles, disorganization of the germinal epithelium, and degenerative changes in some seminiferous tubules as well as its effect on the embryos, therefore, more efforts should be conducted to protect our environment and health from these detrimental compounds and the search for safe methods for insect control.

اختبار فعالية المستخلص التربيني لثمار كل من نبات السباحية ونبات الهوهوبا كطارد ومانع لوضع إناث ذبابة البطيخ الكبرى بيضها داخل ثمار الكوسة

المجلة: مجلة العلوم البحثية والتطبيقية

سنة النشر: 2018

تاريخ النشر: 0018-02-22

The methanolic terpenoid extracts of Sabahaia Plant (Melia azedarach L) and Jojoba Plant (Simmondsiachinensis) were tested under laboratory conditions for oviposition deterrent and repellent activities against the Melon Fly (Dacus frontalis) in pumpkin. Fruits. The extracts were obtained by evaporator system, 10%, 16% and24% concentrations were prepared from Sabahaia plant, and 10%, 15% and 20% concentrations were preparedfrom jojoba plant, and the pumpkin fruits were treated with the two extracts. The results showed that theaverage pupae weight from fruits treated with Sabahia plant terpenoid extract were as follows: 0.015, 0.014 and0.016 gram at the concentration 10%, 16% and 24% respectively, meanwhile, the average pupae weight incontrol was 0.015 gram which shows no significant difference between the used extract concentration and thecontrol. On the other hand, the average pupae weight resulted from pumpkin fruits treated with jojoba plant terpenoid extract concentrations of 10%, 15% and 20% were 0.011, 0.008 and 0.012 gram respectively, there were no significant differences between the used concentrations and the control (untreated fruits), the control average pupae weight was 0.013 gram. ,

اختبار فعالية المستخلص التربيني لثمار كل من نبات السباحية ونبات الهوهوبا كطارد ومانع لوضع إناث ذبابة البطيخ الكبرى بيضها داخل ثمار الكوسة

المجلة: مجلة العلوم البحثية والتطبيقية

سنة النشر: 2018

تاريخ النشر: 0018-02-22

The methanolic terpenoid extracts of Sabahaia Plant (Melia azedarach L) and Jojoba Plant (Simmondsiachinensis) were tested under laboratory conditions for oviposition deterrent and repellent activities against the Melon Fly (Dacus frontalis) in pumpkin. Fruits. The extracts were obtained by evaporator system, 10%, 16% and24% concentrations were prepared from Sabahaia plant, and 10%, 15% and 20% concentrations were preparedfrom jojoba plant, and the pumpkin fruits were treated with the two extracts. The results showed that theaverage pupae weight from fruits treated with Sabahia plant terpenoid extract were as follows: 0.015, 0.014 and0.016 gram at the concentration 10%, 16% and 24% respectively, meanwhile, the average pupae weight incontrol was 0.015 gram which shows no significant difference between the used extract concentration and thecontrol. On the other hand, the average pupae weight resulted from pumpkin fruits treated with jojoba plant terpenoid extract concentrations of 10%, 15% and 20% were 0.011, 0.008 and 0.012 gram respectively, there were no significant differences between the used concentrations and the control (untreated fruits), the control average pupae weight was 0.013 gram. ,

Effect of boiled Peganum harmala seeds on the embryos of experimental laboratory mice

المجلة: مجلة العلوم البحثية والتطبيقية

سنة النشر: 2017

تاريخ النشر: 2017-01-10

Abstract This study was conducted on 120 pregnants Swiss albino mice, their ages 3-4 monthsand their body weights were 25-30 gm. The aim of this study is to detect the effect of boiledharmal seeds infusion on the embryos, particularly during the period of organogenesis. Thepregnants were divided into 6 groups. The pregnants of the first group were given distilled wateronly and considered to be a control group, while the rest groups were orally given the doses 0.5,1, 2, 4 and 6 g / kg of body weight daily, during the days 7-12 of pregnancy. The results showthat the boiled harmal seeds infusion has poison and malformation effects on embryos whosemothers were given doses 1,2,4 and 6 g/kg of body weight, while those of mothers given 0.5 g/kg of body weight appeared normal as those of control group. All of those observed deformativemay be occurred due to the timing of doses given, where the period of organogenesis isconsidered to be the most critical period of pregnancy, so we advise the pregnants to avoidtaking any parts of this plant especially during the early of pregnancy

EFFECT OF TWO DOSES OF THIMATATE PESTICIDE ON THE EMBRYOS OF EXPERIMENTAL LABORATORY MICE

المجلة: مجلة العلوم الاساسية والتطبيقية

سنة النشر: 2013

تاريخ النشر: 2013-03-03

n this study, the effect of two doses of thimatoate pesticide on theembryos of experimental laboratory mice was tested. The work wascarried out on 18 pregnant mothers at 22-25 gram .Female mice were divided into three groups, each of them includessix dams. The first group was given distilled water only, while the tworemaining groups were given daily oral doses of 0.75 and 1.5 ml/kg ofbody weight, respectively, during the days 7-12 of pregnancy.The results of the present study revealed that the recommended dose ofthimatoate pesticide has toxicological and teratogenic effects on theembryos from treated mothers comparing with control group

Ciprofloxacin Affect Sperm Quality and Induced Testicular and Hepatic Injury in Male Mice

المجلة: AlQalam Journal of Medical and Applied Sciences

سنة النشر: 2024

تاريخ النشر: 2024-12-07

Ciprofloxacin (CPFx) is one of a second-generationfluoroquinolones antibiotic and is prescribed a lotfor curing bacterial infection. The aim of this studywas to investigate of impact CPFx on spermcharacteristics, liver DNA and histological structureof liver and testes. Twenty-four male mice aged 7-10weeks were divided into three groups of eight. Thefirst group (control) was given distilled water. Thesecond and third groups received CPFx with doses500 and 750 mg/kg respectively. All treatments wereadministrated orally for 14 days. Three untreatedfemales were put with one treated male for mating.At the end of dosing period, mice were killed, testesand liver were removed and weighed. Spermparameters, testosterone level and liver DNA weremeasured. Histopathology of liver and testes wascarried out. The results showed that CPFxsignificantly affected liver DNA, sperm motility andmorphology as well as it caused histopathologicalalterations of hepatic and testicular tissues.Moreover, it led to a significant elevation in thepercentage of dead fetuses and also deformityfetuses. The results indicate that CPFX hasdetrimental effects on sperm quality, liver DNA andhistological structure of testes and liver; therefore, itshould be used with great caution