la chebula extracts Molecular and Immunological activity of Termina

المجلة: Journal of Advanced Biotechnology and Experimental Therapeutic

سنة النشر: 2022

تاريخ النشر: 2022-01-01

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Immunological and molecular diagnosis of cytomegalovirus infection between aborted & pregnant women in Babylon City

المجلة: Baghdad Science Journal

سنة النشر: 2021

تاريخ النشر: 2021-06-20

Human cytomegalovirus (CMV) is the globally highly prevalent herpesvirus worldwide. CMV infects populations of all ages according to the Center for Disease Control and Prevention (CDC) and World Health Organization (WHO). CMV infections remain the most common viral complication potentially multiple in humans and are a major cause of congenital normality in women, which is why they are critical for diagnosis in several times when it happens during pregnancy. Pregnant women with CMV infection can be in charge of abortion or congenital expandaedby. This study involves the collection a total of (90) samples taken from each aborted and pregnant woman (70 with abortion cases and 20 of pregnant without history of abortion as control subjects) referring to Babylon teaching hospital for Maternity and Children, covering a period from (October 2018 to March 2019) to investigate the occurrence of Cytomegalovirus (CMV) in Babylon city. Patients and controls were evaluated for IgG, IgM antibodies and anti-HCMV IgG, IgM for (90) subjects were controlled in this study using the Enzyme Immunoassay Test Kit and read by enzyme - linked immunosorbent analyze (ELISA). In addition the polymerase chain reaction (PCR) DNA detection for CMV are based on the amplification of pathogen genomes in a particular region using different primers. The Chi-square test was used to analyze the data. The results show among 90 samples, women were evaluated for CMV infection, the seroprevalence titer was significantly higher at P <0.05 in seropositive cases ranging from 62(89%) toward positive CMV IgG, while the 65 (93%) of patients were positive CMV IgM from (70) women with abortions. By contrast, the results obtained from the controls were 9 (45%) subjects seropositive for IgG and all of them were seronegative with IgM. The anti – HCMV IgG finding showed high positivity that represents the furthermost of CMV infections among females through ages ranging between 20-29 years. Furthermore, the outcomes of molecular detection showed that a small number of samples 13 (19%) were HCMV DNA detectable in aborted women less than in pregnant women 3 (15%).

Molecular and Nanotechnical Study for Antibiofilm Formation and CsuE Gene Expression Activities of Synthesized Iron Oxide Nanoparticles Against Multidrug-Resistant Acinetobacter baumannii Isolates

المجلة: Iraqi Journal of Biotechnology

سنة النشر: 2019

تاريخ النشر: 2019-05-21

This study aimed to investigate the influence of synthesis of Iron Oxide Nanoparticles (Fe3O4NPs) by co-precipitation method on biofilm formation and CsuE gene expression in multidrug resistance Acinetobacter baumannii (MDRA.b) which represents one of the important causing agents of nosocomial infection. A. baumannii isolates used in current study and isolates from different clinical sources (wounds, burns, urine, sputum, blood and throat) that able to produce strong biofilm. Synthesis and characterisation the physical-chemical nature of Fe3O4 NPs was carried out using UV-visible spectrophotometer, fourier transform infrared (FT-IR), X-ray diffraction (XRD), Atomic force microscope (AFM) and scanning electron microscope (SEM). Results show that they were very fine crystalline sizes reaching to 11±1 nm by XRD ,with a mostly spherical in shape and average of nanoparticles size between (40- 47) nm by SEM and AFM, respectively. Minimum inhibitory concentration (MIC) and sub-MIC test of Fe3O4 NPs at concentrations (15.75 to 2000) μg/ml against A.baumannii isolates had been determined using tube broth method measured by optical densities values at 630 nm. The results of the experiment of the best sub-MIC with concentration of Fe3O4 NPs 125μg/ml showed a significant difference at p˃0.05 the anti-biofilms inhibitory on polystyrene surface of microtiter plates for the bacteria isolate under study. Biofilm formation CsuE gene expression was investigated by using RT-qPCR technique with reference 16SrRNA gene before and after treatment with Fe3O4 NPs. The results showed a significant difference at p˃0.05 in Cycle threshold (Ct) values for CsuE gene expression of the isolates. The study concludes that the synthesized magnetic Fe3O4 NPs with 125µg/ml gave excellent antibiofilm activities and inhibitory efficiency against A.baumannii isolates and the potentiate to down regulate of gene expression fold for biofilm formation encoded by CsuE gene of A.baumannii leading as a result to have low biofilm production.

Influence of Cigarette Smoking on Liver Function and Lipids Levels Tests: A comparative Study Directed among Smokers and Non-smokers in Babylon Governorate

المجلة: Journal of Biotechnology Research Center

سنة النشر: 2021

تاريخ النشر: 2021-12-21

Cigarette smoking currently is considered as one of the greatest problems in public health worldwide and is risk factor for peripheral vascular disorders and heart disease. The monitoring of liver function and total cholesterol are very important to give an estimation of the future cardiovascular diseases among smokers. The objective of this study is to evaluate the effect of cigarette smoking on the serum levels of liver enzymes: aspartate aminotransferase (GOT), alanine transaminase (GPT) and cholesterol activities among Iraqi smokers in Babylon City, compared to apparently healthy individuals (non-smokers) as a control group (all were males). A case- control study was carried out on forty Iraqi male smokers who smoke at least 10 cigarettes per day for at least15 years. The group includes smokers with age range between 15-55 years. Non-smokers, (control, n= 20) group were collected with the same range of age for statistical comparison. The whole blood samples were drawn by venipuncture from each individual; levels of cholesterol and liver functions test were estimated in the blood serum of smokers and non-smokers by diagnostic kit (Randox corporation-UK) using automatic analyzer.The findings of this study showed that there was a significant higher level of total cholesterol, ALT, and AST in the smokers group compared to non-smokers (P<0.05). As well as, the results showed a significant positive correlation between the smokers’ age and serum GOT, GPT and cholesterol activity (Increasing) especially in age between 45 to 55 years old, as compared to control. In addition, total cholesterol and liver function enzymes were significantly positive correlated with the duration of smoking (P<0.05).Cigarette smoking leads to oxidative stress by free radical generation by the mechanism of lipid peroxidation that is affected by the heaviness of smoking. Smoking exerts negative influence on liver functions test that should be carefully interpreted and preventive strategies needed to avoid the future cardiovascular diseases.