منصة إيفاد العلمية

آيات صلاح الدين محمد حسن

إرسال رسالة

التخصص: باثولوجيا اكلينيكية

الجامعة: معهد تيودور بلهارس للأبحاث

النقاط:

معامل الإنتاج البحثي

الخبرات العلمية

Stem cell culture and isolation
Electron microscopic study of cells

الأبحاث المنشورة

Liver Macrophage Depletion Ameliorates The Effect of Mesenchymal Stem Cell Transplantation in a Murine Model of Injured

المجلة: Scientific Reports

سنة النشر: 2019

تاريخ النشر: 2024-10-19

Abstract Mesenchymal stem cells (MSCs) therapy show different levels of effectiveness in the context of different types of liver damage, suggesting that the microenvironment of the injured liver is a key determinant for effective stem cell therapy. The objective was to assess the modulatory effect of hepatic stem cell niche components on the transplanted MSCs during liver injury induced by carbon tetrachloride (CCl4). Superparamagnetic iron oxide (SPIO)-labeled human MSCs were injected intravenously into mice treated with CCl4 and subjected to hepatic macrophage-depletion. Liver tissues were collected at different intervals post transplantation for subsequent histopathological, morphometric, immunohistochemical, gene expression and ultrastructural studies. The homing of the transplanted MSCs was evidenced by tracing them within the niche by iron staining and immunohistochemical studies. MSCs differentiated into hepatocyte-like cells and intimal smooth muscle cells as evidenced by their expression of human albumin and α-smooth muscle actin with a concomitant increase in the level of mouse hepatocyte growth factor. A post transplantation reduction in the liver fibro-inflammatory reaction was found and was promoted by liver macrophages depletion. Thus, it could be concluded from the present study that prior manipulation of the microenvironment is required to improve the outcome of the transplanted cells.

Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases

المجلة: Journal of digestive disease

سنة النشر: 2012

تاريخ النشر: 2024-10-19

OBJECTIVE: The present study was aimed to investigate and compare the kinetics of bone marrowderived hematopoietic stem cells (BMHSC) migration in the peripheral blood and liver in response to liver injury in patients with chronic liver disease (CLD). METHODS: In all, 45 CLD patients staged with Child–Pugh A, B and C and 15 healthy participants were evaluated for the concentration of circulating BMHSC by a flow cytometric analysis of CD133+/ CD34+ cells. In addition, homing BMHSC and hepatic progenitors were assessed by the immunohistochemical detection of CD133+ and OV6+ cells in liver biopsy specimens from Child–Pugh A and B patients. RESULTS: No significant difference in the percentage of circulating CD133+/CD34+ cells was observed among all groups of patients. In liver tissues, OV6+ cells increased significantly in Child–Pugh B cases (P < 0.05), while CD133+ cells were distributed sparsely in the periportal region in Child–Pugh A and B patients. OV6+ cells were significantly correlated with CD34+ cells but not with CD133+ cells in Child–Pugh A and B patients (P < 0.01 and P < 0.05, respectively). CONCLUSIONS: Various degrees of severity in CLD neither evoked the mobilization of BMHSC into the circulation nor triggered their homing into liver tissue, thus excluding extrahepatic stem cell-mediated repair. The recovery process seems to be dependent on proliferating endogenous liver progenitors (OV6+ cells).

Procoagulant activity of red blood cell microparticles in stored packed red blood cell units and its relation to ABO blood grouping

المجلة: Beni-Suef University Journal of Basic and Applied Sciences

سنة النشر: 2024

تاريخ النشر: 2024-06-04

Background Throughout the storage of blood, the red cells undergo alterations known as “storage lesions,” which involve shape changes and the formation of microparticles (MPs). Studies of the formation of red cell microparticles (RMPs) emphasize the prospective application of RMPs as a quality control measure in the preparation and storage of blood components in the future. In the present study, twenty packed RBC units in citrate phosphate dextrose adenine-1 (CPDA1) were collected from volunteers and stored for 35 days. Over 35 days of storage, samples were collected at six distinct time points weekly and evaluated for the presence of RMPs. MPs were separated by the ultracentrifugation method. Electron microscopy was used to characterize the morphology and size of the isolated microparticles, and flow cytometry was performed to determine the percentage of RMPs that expressed glycophorin A (CD235a) and Annexin V antigens. RMPs' procoagulant activity (PCA) was assessed using a plasma recalcification test. RMP concentration in accordance with ABO blood grouping was assessed by using various types of donated blood groups. Results RMPs progressively increased over storage. The procoagulant activity (PCA) exhibited a significant increase during storage, as evidenced by a shorter plasma recalcification time (P value = 0.001). A significant negative correlation (P value = 0.001) between plasma recalcification time and Annexin V-positive microparticles, as well as a dual-positive Annexin V/CD235a population, was identified, indicating a strong correlation between the direct quantitative assay by flowcytometry and the functional assay through the PCA. Conclusion RMPs increase on storage with increased PCA. Finding ways to reduce these microparticles in packed RBC units is crucial for reducing the risk of transfusion-related coagulopathy.

Ultrastructural changes of platelets in COVID-19 and chronic viral hepatitis patients

المجلة: Ultrastructural pathology

سنة النشر: 2024

تاريخ النشر: 2024-04-09

Abstract Platelet-viral interactions are evolving as a new concern. Coagulation disorder is a major consequence of the COVID-19 infection. In chronic hepatitis virus infections, defect in coagulation factors, thrombocytopenia and platelet function abnormalities are common. A SARS-CoV-2 infection on top of chronic viral hepatitis infection can be common in areas where viral hepatitis is endemic. Here, we investigate the platelet ultrastructural changes and estimate the serum platelet factor-4 (PF-4), ferritin, CRP, and D-dimer in COVID-19 patients (n = 60), COVID-19 patients with associated chronic viral hepatitis (n = 20), and healthy subjects (n = 20). Ultrastructural changes were demonstrated in all test groups, denoting platelet activation. In chronic viral hepatitis patients, Platelet ultrastrustural apoptotic changes were also seen. Significantly high levels of PF-4 were confirmed in moderate and severe COVID-19 patients (P.value <0.001), with a cut off value of 17 ng/ml for predicting disease severity. A positive correlation of PF-4 with the level of serum ferritin, CRP, and D-dimer (p value < 0.001) was noted, while negatively correlated with platelet count and platelet granule count (p value < 0.001). In our study, chronic viral hepatitis patients presented mild COVID-19 signs, and their PF-4 level was comparable with the subgroup of mild COVID-19 infection. The platelet's critical role in COVID-19 coagulopathy and chronic viral hepatitis is evidenced by the ultrastructural changes and the high levels of PF4. Moreover, a dual viral infection poses a substantial burden on the platelets, necessitating close monitoring of the patient's coagulation profile. Keywords: COVID-19; Chronic viral hepatitis; Platelets activation.

Micro RNA Let7c Induced Monocyte Activation in Patients with Chronic HCV Induced HCC Via Regulation of IL10

المجلة: African journal of biological science

سنة النشر: 2024

تاريخ النشر: 2024-09-05

Background Monocytes, critical components of the immune system, act as a link between innate and adaptive immunity. In the context of chronic hepatitis C virus infection, progressing to liver cirrhosis and hepatocellular carcinoma (HCC), monocytes differentiation into macrophages is thought to be crucial in altering microenvironment of the tumor. Recent data emphasizes the critical role of microRNAs (miRNAs) in the regulation of gene expression, influencing monocyte activation, immune responses, and cancer progression. Chronic liver disorders, such as HCV-related cirrhosis and HCC, have been linked to the dysregulation of certain miRNAs. Particularly miRNA let-7c has attracted attention because of its role in immune regulation and tumor biology. Objectives Here, we investigated the role of miRNA let-7c in controlling monocyte activation in chronic HCV-related cirrhosis and HCC, with an emphasis on its relationship to the immune-regulatory cytokine IL10. Methods The study included 54 patients with chronic HCV infection, divided into 3 groups; chronic HCV, liver cirrhosis, and HCC, as well as, 15 healthy individuals as controls. MiRNA let-7c and IL10 gene expression were assessed by RT-PCR, and CD16 and CX3CR1 expression on monocytes was assessed by flowcytometry. Results: The results showed that whilst IL10 expression was significantly elevated in HCC patients in comparison to HCV, cirrhosis and controls (P-value < 0.05), miRNA let-7c expression was gradually downregulated from chronic HCV to cirrhosis and HCC (P-value < 0.01). Furthermore, the percentage of CD16+CX3CR1+ monocytes was significantly increased the disease with disease severity, indicating their potential role in HCC progression. Conclusion Our findings suggest that decreased miRNA let-7c expression may contribute to monocyte activation and immune modulation in HCC via the regulation of IL10. IL10 overexpression in HCC may facilitate immune evasion and tumor progression, making it a promising biomarker for HCC diagnosis and monitoring. miRNA let-7c and CD16+CX3CR1+ monocytes could be valuable biomarkers for following the development of cirrhosis and HCC from a persistent HCV infection. Targeting the IL10 pathway could offer new therapeutic strategies for HCC treatment.

Micro RNA 155 and Its Target Gene CTLA4 As a New Biomarker for Lymphocyte ‎Activation in HCV-Induced Liver Fibrosis and Hepatocellular Carcinoma

المجلة: Egyptian journal of chemistry

سنة النشر: 2024

تاريخ النشر: 2024-10-13

The role of microRNA 155 (miRNA 155) and the immunological checkpoint cytotoxic T-lymphocyte associated antigen 4 ‎‎(CTLA-4) in chronic liver diseases and hepatocellular carcinoma (HCC) are complex. The alterations in the expression levels or ‎functions of CTLA-4 and miRNA 155 could influence the outcome of HCV infection by affecting the quality and persistence of ‎the antiviral immune response. Here, we tried to investigate the relationships of miRNA 155 gene expression with CTLA-4, the ‎regulator gene of lymphocyte activation in patients with HCC and chronic HCV infection to assess the interactions between these ‎genes and lymphocyte activation and its contribution in liver disease progression. A case-control study included 80 subjects (20 ‎healthy controls, 20 HCV patients without cirrhosis, 20 with liver cirrhosis, and 20 patients with HCC). The expression of ‎miRNA 155 and CTLA-4 gene were measured by quantitative real-time qRT-PCR. The percentage of circulating regulatory T (T ‎reg) cells expressing CD4 and CD25 was done by flow cytometry. Results revealed that miRNA 155 and CTLA-4 gene were ‎upregulated with higher expression in HCC and liver cirrhosis patients compared to HCV without liver cirrhosis and control ‎groups (p value < 0.05). Together with a significant increase in CD4+CD25+ T reg in HCC and liver cirrhosis patients ‎compared to the HCV group (p value <0.05). In conclusion, miRNA 155 and CTLA-4 gene could be utilized as new ‎biomarkers in HCV- induced liver fibrosis and HCC for further exploration of immunomodulatory strategies targeting these ‎molecules to enhance antiviral immune responses and prevent hepatocarcinogenesis.‎

Phonotyping Transition of Infiltrating Hepatic Monocytes to Fibroblasts In CHC Patient's Induced Liver Cirrhosis

المجلة: International journal of chemical and biochemical science

سنة النشر: 2023

تاريخ النشر: 2023-12-20

Monocytes differentiate into other cell types, including fibroblasts which infiltrate the damaged tissue and participate in the fibrotic processes. We aim to assess the possible contribution of circulating monocytes and infiltrating hepatic monocytes in hepatitis C virus (HCV)-induced expansion of the myofibroblastic pool resulting in liver fibrosis. 105 subjects with HCV-induced chronic liver disease were grouped into three groups according to METAVIR score. And assessed by Lab investigations, abdominal ultrasonography, CT and Ultrasound-guided liver biopsy for histopathological and immunohistochemical studies. Flow cytometric and immunohistochemical analysis of (circulating and tissue) monocyte markers using anti- (CD45, CD14, and CD16 and α-SMA). Determination of serum levels of α-SMA and TGF-β was also conducted using ELISA. CD14+, CD16+ and α-SMA+ Cells were upregulated with the increase in hepatitis and fibrosis grade activity (p< 0.01). CD45+cells showed higher expression in grades II&III fibrosis compared to grade I. however,CD45+ cells was not correlated with the stage of hepatic fibrosis. CD14+, CD16+ and α-SMA+ cells were significantly elevated in cirrhotic tissues compared to non-cirrhotic. A significant up regulation of CD14+ CD16+ monocytes observed in circulation with increased serum levels of α-SMA and TGF-β along with the down regulation of CD14+ monocytes which paralleled the severity of liver disease and the progression of liver fibrosis. Infiltrating hepatic monocytes may have undergone a phenotypical transition to fibroblasts. CD14, CD16, and α-SMA can serve as predictive biomarkers, and further measurement of their soluble forms can be used as a non-invasive tool for the diagnosis of progression of liver fibrosis.

The Role of MicroRNAs (miRNA 155, miRNA-146b) and Procalcitonin as Novel Markers for the Diagnosis of Spontaneous Bacterial Peritonitis

المجلة: The open biomarkers journal

سنة النشر: 2021

تاريخ النشر: 2021-04-15

tonitis in cirrhotic liver patients. miRNA-155 and 146b are molecular biomarkers , while procalcitonin is a serum marker in ascites patients complicated with Spontaneous Bacterial Peritonitis (SBP) . Methods: This study was conducted on 199 patients, 101 of them have ascites complicated with spontaneous bacterial peritonitis, and 98 patients without spontaneous bacterial peritonitis (control group). Ascitic fluid samples were collected from patients with SBP undergoing paracentesis at National Hepatology Institute in Egypt. MicroRNAs were determined in the serum using qPCR (quantitative polymerase chain reaction), while procalcitonin has been assessed in serum samples using ELISA (Enzyme-linked immune assay) technique. Results: Serum levels of miRNA-146b & miRNA-155 were significantly higher (p<0.001) in spontaneous bacterial peritonitis patients (79.2% and 97.0% respectively) than ascites patients (17.3% and 7.1%, respectively). Furthermore, the serum level of procalcitonin was significantly higher (p<0.001) in spontaneous bacterial peritonitis patients than that in ascites patients (68.3% and 27.6%, respectively). Conclusion: miRNA-155, miRNA-146b and procalcitonin can be used as early markers for the detection of SBP in hepatic patients with ascites.