منصة إيفاد العلمية

اسماء وجيه زغلول

إرسال رسالة

التخصص: استاذ مساعد

الجامعة: الزقازيق

النقاط:

معامل الإنتاج البحثي

الخبرات العلمية

1- الاشراف الاكاديمى على الرسائل العلميه
2- تقييم الابحاث العلميه للنشر فى المجلات الدوليه والمحليه
3- المشاركه فى المحاضرات والندوات والمؤتمرات وورش العمل
4- عضو هيئه تحرير فى مجلات علميه محليه ودوليه

الأبحاث المنشورة

Partial defatted black solider larvae meal as a promising strategy to replace fish meal protein in diet for Nile tilapia (Oreochromis niloticus): Performance, expression of protein and fat transporters, and cytokines related genes and economic efficiency

المجلة: Aquaculture

سنة النشر: 2022

تاريخ النشر: 2022-03-24

Insect meal can be a promising nutrients source in aquafeed, replacing expensive, scarce or unsustainable foods however; molecular mechanisms behind its role on fish growth remain unknown. From this point, the impact of partial or total replacement of fish meal protein with defatted black soldier larvae meal (PD-BSLM) on Nile tilapia’s performance, body composition, expression of genes related protein and fatty acids transporters and immunity and economic efficiency was evaluated. A total of 500 Nile tilapia (17.9 ± 0.3 g) was distributed into 4 groups: control fed basal diet with 10% fish meal; the other 3 groups were fed basal in which fish meal was replaced by 25, 50 and 100% of PD-BSLM for 12-weeks. The results described that dietary inclusion of PD-BSLM had no negative impact on Nile tilapia’s growth rate, feed conversion ratio, protein utilization and nutrient digestibility even after 100% replacement. Body fat content was significantly decreased with increasing PD-BSLM levels. Amino acids analysis of fish body showed that replacement of fish meal with 100% PD-BSLM has a slight change on its lysine content. Interestingly, lysozymes, IgM and nitric oxide levels were significantly enhanced in group fed PD-BSLM at 100% replacement level. Transcriptomics profile of genes encoding amino acids (SLC3A1- SLC6A19) and peptides transporters (SLC15a1a-SLC15a1b-SLC15a2) revealed no negative effect in response to PD-BSLM. Moreover, mRNA expression of genes controlling fat transportation and metabolism (FABP-2, PPAR-α and PPAR-β) was up-regulated in groups fed higher PD-BSLM levels. Remarkably, mRNA expression of proinflammatory cytokines (IL-1β gene TNF-α) was down-regulated and anti-inflammatory cytokines (IL-10) was upregulated at higher PD-BSLM levels. Therefore, our study reported that replacement of fish meal with PD-BSLM up to 100% had a positive modulating effect on the expression of protein and fat transporters that can explain a non adversely affected growth performance of Nile tilapia. Besides, the higher inclusion levels of PD-BSLM can boost immunity of Nile tilapia as well as increased the economic efficiency

Black Pepper or Radish Seed Oils in a New Combination of Essential Oils Modulated Broiler Chickens’ Performance and Expression of Digestive Enzymes, Lipogenesis, Immunity, and Autophagy

المجلة: vet.sci

سنة النشر: 2022

تاريخ النشر: 2022-01-24

Optimal combinations of essential oils (EOs) can enhance performance and maintain poultry productivity. The effects of EOs with black pepper oil (BPO) or radish seed oil (RSO) on performance and the expression of digestive enzymes, lipogenesis, immunity, and autophagy-related genes in broiler chickens were explored. Six dietary treatments for 300 one-day-old chicks were allocated as follows: controls were fed a basal diet, one group was fed an EO-supplemented diet (1.5 g/kg diet of parsley, mint, and carrot seed oils (1:1:1)), and other groups received Eos + BPO0.25, Eos + BPO0.5, Eos + RSO0.25, and Eos + RSO0.5 treatments, with a basal diet containing EOs plus BPO or RSO at the level of 0.25 or 0.5 g/kg, respectively. Supplementation with 0.5 g/kg of EOs plus BPO or RSO resulted in the most improved maximum BWG and FCR in broiler chickens. The lactobacilli population was increased in Eos + BPO0.5, followed by EOs + RSO0.5, unlike in the control. The highest expression of the CCK and PNLIP genes was identified in the Eos + BPO group. The FAS and ACC genes were upregulated, while the IgA and IL-10 genes were downregulated, with EOs plus RSO or BPO. The group that received Eos + BPO0.5, followed by Eos + RSO0.5, displayed patterns of higher expression for atg5, atg7, and atg12, with lower expression of mTOR. In summary, a new combination of EOs with 0.5 g/kg BPO had potential growth-promoting and immune-boosting effects in broiler chickens.

Impact of resveratrol-loaded liposomal nanocarriers on heat-stressed broiler chickens: Eects on performance, sirtuin expression, oxidative stress regulators, and muscle building factors

المجلة: frontiers

سنة النشر: 2023

تاريخ النشر: 2023-03-28

Climate change is considered to be the primary cause of heat stress (HS) in broiler chickens. Owing to the unique properties of extracted polyphenols, resveratrol-loaded liposomal nanoparticles (Resv-Lipo NPs) were first explored to mitigate the harmful eects of HS. The dietary role of Resv-Lipo NPs in heat-stressed birds was investigated based on their growth performance, antioxidative potential, and the expression of heat shock proteins, sirtuins, antioxidant, immune, and muscle-building related genes. A total of 250 1-day-old Ross 308 broiler chickens were divided into five experimental groups (5 replicates/group, 10 birds/replicate) for 42 days as follows: the control group was fed a basal diet and reared in thermoneutral conditions, and the other four HS groups were fed a basal diet supplemented with Resv-Lipo NPsI, II, and III at the levels of 0, 50, 100, and 150 mg/kg diet, respectively. The results indicated that supplementation with Resv-Lipo NP improved the growth rate of the HS group. The Resv-Lipo NP group showed the most significant improvement in body weight gain (p < 0.05) and FCR. Additionally, post-HS exposure, the groups that received Resv-Lipo NPs showed restored functions of the kidney and the liver as well as improvements in the lipid profile. The restoration occurred especially at higher levels in the Resv-Lipo NP group compared to the HS group. The elevated corticosterone and T3 and T4 hormone levels in the HS Frontiers in Veterinary Science 01 frontiersin.orgKishawy et al. 10.3389/fvets.2023.1137896 group returned to the normal range in the Resv-Lipo NPsIII group. Additionally, the HS groups supplemented with Resv-Lipo NPs showed an improvement in serum and muscle antioxidant biomarkers. The upregulation of the muscle and intestinal antioxidant-related genes (SOD, CAT, GSH-PX, NR-f2, and HO- 1) and the muscle-building genes (myostatin, MyoD, and mTOR) was observed with increasing the level of Resv-Lipo NPs. Heat stress upregulated heat shock proteins (HSP) 70 and 90 gene expression, which was restored to normal levels in HS+Resv-Lipo NPsIII. Moreover, the expression of sirtuin 1, 3, and 7 (SIRT1, SIRT3, and SIRT7) genes was increased (p < 0.05) in the liver of the HS groups that received Resv-Lipo NPs in a dose-dependent manner. Notably, the upregulation of proinflammatory cytokines in the HS group was restored in the HS groups that received Resv-Lipo NPs. Supplementation with Resv-Lipo NPs can mitigate the harmful impact of HS and consequently improve the performance of broiler chickens.

Effect of parsley essential oil on digestive enzymes, intestinal morphometry, blood chemistry and stress-related genes in liver of Nile tilapia fish exposed to Bifenthrin

المجلة: Aquaculture

سنة النشر: 2021

تاريخ النشر: 2021-08-10

Bifenthrin (BF), third-generation synthetic pyrethroids (SPs), is extensively used as an insecticide for its higher killing activity, upgraded stability, and lower mammalians toxicity, compared to earlier SPs. The monitoring of high concentrations of BF in aquatic environment addresses a significant concern to aquatics including fish. In the current investigation, O. niloticus fish was subjected to bifenthrin insecticide (BF) at a dose level equal to 1/10 LC50 (0.68 μg/L) for a period of successive 60 days, followed by assessing of BF-induced hepatotoxic and digestive insults through the biochemical and transcriptomic changes. Moreover, the efficiency of parsley (Petroselinum crispum) essential oil (PLO) in two dose levels to mitigate these impacts was evaluated. Our findings showed that BF induced a high rate of mortality and altered the total body composition, serum biomarkers of hepatorenal function and liver glycogen. Intestinal morphological changes were recorded as well as suppression of activity of the intestinal α-amylase, lipase and protease. Oxidative injury was asserted by the recorded elevation of hepatic malondialdehyde (MDA), protein carbonyl, accompanied with depletion of the content of GSH, and repression of the activity of the both antioxidant enzymes (SOD and CAT). In addition, the RT-PCR analysis of stress-mediated genes showed an upregulated expression manner of tumor suppressor protein (P53), Caspase-3, and heat shock protein-70 (HSP-70) in hepatic tissue. Concurrent supplementation of PLO at the both levels in the diet with BF intoxication modulated the recorded deleterious influences in a dosedependent way. The data from the current study stated the induction of hepatotoxicity and digestive disorders due to BF exposure in O. niloticus fish, and approved the protective efficacy of PLO to be a promising additive to fish feed.

Origanum vulgare Essential Oil Modulates the AFB1-Induced Oxidative Damages, Nephropathy, and Altered Inflammatory Responses in Growing Rabbits

المجلة: toxins

سنة النشر: 2023

تاريخ النشر: 2023-01-12

The current study was performed to investigate the toxic effects of aflatoxin B1 (AFB1) through the evaluation of kidney function tests and histopathological examination of renal tissues, targeting the therapeutic role of Marjoram (Origanum vulgare essential oil-OEO) in improving health status. Forty-eight New Zealand Whites growing rabbits (four weeks old) weighing on average 660.5 ± 2.33 g were randomly and equally distributed into four groups, each of which had four replicas of three animals as the following: Control group (only basal diet), AFB1 group (0.3 mg AFB1/kg diet), OEO group (1 g OEO/kg diet) and co-exposed group (1 g OEO/kg + 0.3 mg AF/kg diet). Our study lasted eight weeks and was completed at 12 weeks of age. The results revealed that OEO decreased the toxic effects of AFB1 in rabbit kidneys by substantially reducing the cystatin C levels in the AFB1 group. Additionally, OEO decreased oxidative stress and lipid peroxidation levels in the co-exposed group. Moreover, OEO reduced DNA damage and inflammatory response in addition to the down-regulation of stress and inflammatory cytokines-encoding genes. Besides, OEO preserved the cytoarchitecture of rabbits’ kidneys treated with AFB1. In conclusion, O. vulgare essential oil supplementation ameliorated the deleterious effects of AFB1 on the rabbits’ kidneys by raising antioxidant levels, decreasing inflammation, and reversing oxidative DNA damage.

Thiacloprid Induced Developmental Neurotoxicity via ROS-Oxidative Injury and Inflammation in Chicken Embryo: The Possible Attenuating Role of Chicoric and Rosmarinic Acids

المجلة: biology

سنة النشر: 2021

تاريخ النشر: 2021-10-25

The current study was designed to evaluate the negative impact of thiacloprid (TH) on the brain tissue of developing chicken embryo models and to evaluate the modulatory effects of chicoric (CA) and rosmarinic (RA) acids. The eggs were injected in ovo with different doses of TH (0.1, 1, 10, and 100 µg/egg). TH significantly increased the oxidative damage in the brain of exposed embryos in a dose-dependent manner (p < 0.001). TH significantly elevated the oxidative stress markers; protein carbonyl, malondialdehyde (MDA) content, and DNA damage (p < 0.001). Myeloperoxidase (MPO) activity and NO significantly increased with overexpression of the pro-inflammatory cytokines (IFN-γ; interferon gamma, TNF-α; tumor necrosis factor alpha, and IL-1β; interleukin-1 beta), stress-related and apoptotic genes (NF-KB, Caspase-3) in the brain tissue on both a biochemical and molecular levels (p < 0.05), while downregulating the expression of antiapoptotic Bcl-2. Co-treatment of CA and RA with TH markedly decreased the insecticide-induced toxicity with a prominent synergistic effect (p < 0.05). In conclusion, TH is suggested to be a possible neurotoxic to embryos of vertebrates and possibly humans. The study also revealed the antioxidant, anti-inflammatory, genoprotective, and antiapoptotic properties of CA and RA against TH toxicity

Imidacloprid toxicity in Clarias gariepinus: Protective role of dietary Hyphaene thebaica against biochemical and histopathological disruption, oxidative stress, immune genes expressions, and Aeromonas sobria infection

المجلة: Aquaculture

سنة النشر: 2022

تاريخ النشر: 2022-03-20

Recently, there has been a controversy about the hazards of toxic substances released to the aquatic biota. Therefore, the current context is the first attempt to assess the ability of a beneficial medicinal plant, Hyphaene thebaica fruit (HTF) to mitigate various toxic impacts in Clarias gariepinus induced by imidacloprid (IMI) including immune depression, protein impairment, oxidative stress, hepato-renal dysfunction, histopathological, and immune-histochemical alterations. The molecular responses of IMI-induced toxicity at the molecular level and to recognize critical genes altered in response to toxicity are documented. Additionally, we investigated the fish resistance post-challenging with a pathogenic zoonotic bacterium, Aeromonas sobria. Fish (N = 240) were randomly allotted into four random groups in triplicate. The first (control) and second (HTF) groups were fed on basal diets that were enriched with 0.0 and 15 g kg− 1 of HTF without IMI exposure. The third and fourth groups were fed on the same diets and exposed to 1/5 of the 96-h of LC50 of IMI (2.03 μg L− 1) for 60 days. The findings clarified that the IMI exposure significantly augmented (p < 0.05) serum alanine (ALT), aspartate aminotransferases (AST), urea, and creatinine besides malondialdehyde (MDA) levels. While the activities of the protein profile (total protein, albumin, and globulin), immune parameters (lysozyme and immunoglobulin M), and hepatic antioxidant enzymes (superoxide dismutase and glutathione peroxidase) revealed marked depressions in comparison to the control. Histological and immune-histochemical alterations in liver and kidney tissues including vacuolation, severe necrosis, and degeneration, besides weak B-cell lymphoma 2 (BCL-2), potent caspase-3, and down-regulation of genes (IL-1β, IL-8, TNF-α, TGF-β, TLR-5, LYSG, LYSC, NF-κβ, CC, and MYE) were noticed in the IMI-exposed group compared to the control. After 60 days of experimental trial, C. gariepinus challenged by A. sobria exhibited sluggish movement, fin rot accompanied with hemorrhages in different body parts, and severe skin ulcerations with the highest cumulative mortality (80%). Surprisingly, HTFdietary inclusion enhanced these variables by almost renovating to control values by maintaining histological architecture of the hepatic and renal tissues. Additionally, it increased the fish resistance against A. sobria indicated by reduced mortalities (40%) and enhanced clinical picture. The outputs of the recent study recommend the HTF-dietary supplement to protect against the IMI toxicity in C. gariepinus. And provide insights into the future application of the HTF as an anti-toxic, immune-stimulant, antioxidant, and antibacterial, for improving fish health and sustaining aquaculture. To avoid the deleterious toxic impacts of IMI, this pesticide must be applied carefully, especially near water bodies.

Palliative role of colloidal silver nanoparticles synthetized by moringa against Saprolegnia spp. infection in Nile Tilapia: Biochemical, immuno-antioxidant response, gene expression, and histopathological investigation

المجلة: aquaculture report

سنة النشر: 2022

تاريخ النشر: 2022-09-05

Our study proposed a promising anti-fungal therapy to control Saprolegnia spp. infection in Nile tilapia (Oreochromis niloticus). A Moringa oleifera synthesized silver nanoparticles (MS-AgNPs) showed an in vitro anti-fungal activity against Saprolegnia spp. with an inhibition zone of 18 mm. A total of 220 O. niloticus (45 ± 0.56 g) were used in this study. A preliminary investigation was conducted by using 100 fish for determining the safe dose of MS-AgNPs which was found to be 0.6 mg/L. Another 120 fish were divided into four groups; the first (control) and second groups (G1 and G2) were non-infected and exposed to 0 and 0.6 mg/L MS-AgNPs, respectively. The third and fourth groups (G3 and G4) were infected with Saprolegnia spp. (1.5 × 104 spores/L) and exposed to 0 and 0.6 mg/L MS-AgNPs, respectively. The experiment lasted for 7 days. The infection with Saprolegnia spp. induced immunosuppression expressed by a marked decrease in immunoglobulin M, nitric oxide, complement 3, and lysozyme activity, with a 41 % survival rate. Additionally, a marked decline in the values of hepatic and branchial antioxidant enzymes (superoxide dismutase and catalase) in addition to serum total antioxidant capacity and protein profile indices. In contrast, Saprolegnia spp. infection elevated hepatic and branchial malondialdehyde, hepatic function indicators, and stress biomarkers (cholesterol and triglyceride) with up-regulation of the pro-inflammatory cytokines (IL-1ß and TNF-α), and the anti-inflammatory cytokines (IL-10 and TGF-β). Histopathological findings of the gills and musculature of infected fish showed severe hyperplasia of the epithelial lining with a fusion of secondary lamellae and necrotic myocytes admixed with edema. Interestingly, treatment of the challenged fish with MS-AgNPs at a dose of 0.6 mg/L could improve these variables by enhancing the histological architecture. According to these findings, MS-AgNPs have an ameliorative effect against the harmful effects of Saprolegnia spp. infection on immune-antioxidant status and hepatic functions indices. Consequently, MS-AgNPs at a dose of 0.6 mg/L is a promising option for use in the control of Saprolegnia spp. infection in aquaculture.

Pseudomonas putida: Sensitivity to Various Antibiotics, Genetic Diversity, Virulence, and Role of Formic Acid to Modulate the Immune-Antioxidant Status of the Challenged Nile tilapia Compared to Carvacrol Oil

المجلة: fishes

سنة النشر: 2022

تاريخ النشر: 2022-12-22

The Pseudomonas putida strain was primarily identified and tested in vitro against antibiotic sensitivity for several antibiotics using the disc diffusion method. This isolate was also tested against sensitivity to carvacrol oil (c) and formic acid (f). The genotyping of Pseudomonas spp. and virulotyping for P. putida isolate was carried out and verified by 16S rDNA-PCR amplification. Furthermore, we assessed the efficacy of carvacrol oil and formic acid in vivo for treatment of P. Putida infection. For the in vivo challenge, 180 fish (Nile tilapia, Oreochromis niloticus) were divided into six groups: (G1: control (unchallenged), G2: carvacrol prophylaxis (3 g/kg), G3: formic acid prophylaxis (5 mL/kg), G4: control positive (challenged), G5: carvacrol treatment (3 g/kg), and G6: formic acid treatment (5 mL/kg); 30 fish per group) with three replicates. Following the challenge, nitric oxide and lysozyme activity were measured as essential indicators for fish immunity. The antioxidant parameters including SOD and catalase were computed to reflect the antioxidant status. Furthermore, relative percent survival (RPS) and mortality percent were evaluated to indicate functional immunity. The findings of the antibiotic sensitivity test showed that ciprofloxacin exhibited the largest inhibition zone. Additionally, formic acid (f) displayed the greatest inhibition zone compared to carvacrol oil (c) and was more effective in stimulating the immune-antioxidant response compared to carvacrol oil. The tested exotoxin A (tox A), exoenzyme S (exo S), and the nan1 associated-virulence genes were identified in the P. putida isolate. Overall, the current study verified the virulence of P. putida and highlighted the promising role of dietary addition of formic acid for enhancing the immuneantioxidant indicators and for mitigating P. putida infection. Future studies could be devoted to this field.

Thermal stress consequences on growth performance, immunological response, antioxidant status, and profitability of finishing broilers: transcriptomic profile change of stress-related genes

المجلة: Tropical Animal Health and Production

سنة النشر: 2020

تاريخ النشر: 2020-09-25

The current study was conducted to investigate the impact of thermal stress on growth performance, blood biomarkers, metabolic hormones, immunological response, antioxidant activity, and expression of stress-related genes in broilers. One hundred and fifty one-day-old chicks (Ross 308) were utilized in this work. On the 21st day of age, birds were subjected to three environmentally controlled treatments with five replicate pens of 10 birds per each, representing an initial density of 10 birds/m2—control: reared in a thermoneutral condition; THS1 and THS2: exposed to 4 and 6 h of daily thermal stress at 40 ± 1 °C, respectively, until the 42 days of age. The results demonstrated that thermal stress for 4 and 6 h significantly reduced (P < 0.001) daily weight gain, daily feed intake, blood leukocyte and lymphocyte counts, serum immunoglobulins (IgM, IgA, IgG), and insulin-like growth factor-1 (IGF-1), while serum levels of aspartate aminotransferase, alanine aminotransferase, glucose, cholesterol, low-density lipoprotein, and lactate dehydrogenase were elevated relative to the thermoneutral group. Additionally, the corticosterone level and the ratio of heterophil:lymphocyte increased significantly (P < 0.001) in thermal-stressed groups. The antioxidant enzymes were affected by thermal stress as represented by a significant decrease in the activity of serum catalase (CAT) and glutathione peroxidase (GSH-Px) along with an increase in malonaldehyde concentration. Thermal stress affected gene expression by upregulating heat shock protein 70, heat shock factors 1 and 3, nuclear factor kappa B, interleukin-4, and uncoupling protein, and downregulating GSH-Px, CAT, and IGF-1 transcript levels. However, no changes were observed in interleukin-2 expression levels. It can be concluded that thermal stress destructively influences productivity, physiological status, and gene expression by upregulating heat shock protein 70, heat shock factors 1 and 3, nuclear factor kappa B, interleukin-4, and uncoupling protein, and downregulating GSH-Px, CAT, and IGF-1 transcript levels of broiler chickens